![]() From the early work developed by Harris et al. Traction Force Microscopy (TFM) is a powerful methodology of quantifying cellular forces during cell-material interactions. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. by NSF CBET 1134166 to D.H.K., and an NSF Graduate Research Fellowship to E.B.K. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.įunding: This work is in part supported by NIH R21 Al101469-01 to C.F. Received: NovemAccepted: FebruPublished: April 16, 2014Ĭopyright: © 2014 Toyjanova et al. PLoS ONE 9(4):Įditor: Igor Sokolov, Tufts University, United States of America Particular attention is paid in estimating the accuracy penalty associated with utilizing a traditional linear elastic approach in the presence of large deformation gradients.Ĭitation: Toyjanova J, Bar-Kochba E, López-Fagundo C, Reichner J, Hoffman-Kim D, Franck C (2014) High Resolution, Large Deformation 3D Traction Force Microscopy. Based on our previous 3D TFM technique, we reformulate our approach to accurately account for large material deformation and quantitatively contrast and compare both linear and large deformation frameworks as a function of the applied cell deformation. The results feature some of the first experimental evidence that cells are indeed capable of exerting large material deformations, which require the formulation of a new theoretical TFM framework to accurately calculate the traction forces. Here we present a new high resolution 3D TFM algorithm which utilizes a large deformation formulation to quantify cellular displacement fields with unprecedented resolution. ![]() Yet irrespective of dimensionality, almost all TFM approaches have relied on a linear elastic theory framework to calculate cell surface tractions. In addition, recent advances in three-dimensional (3D) imaging and traction force analysis (3D TFM) have highlighted the significance of the third dimension in influencing various cellular processes. Traction Force Microscopy (TFM) is a powerful approach for quantifying cell-material interactions that over the last two decades has contributed significantly to our understanding of cellular mechanosensing and mechanotransduction.
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